Does conjugated bilirubin displace unconjugated bilirubin from albumin.

Does conjugated bilirubin displace unconjugated bilirubin from albumin.

Influence of albumin on the hepatic uptake of unconjugated bilirubin.

1. The initial distiibution volumes of [3H]bilirubin in six adult subjects were equivalent to those of "I-labelled albumin following simultaneous intravenous injection of both compounds. However, the initial rate of loss of [3H]bilirubin from plasma was seven to thirty times faster than that of 1311-labelled albumin. 2. In vitro uptake of [3H]bilirubin by Gunn rat liver slices occurred at a rat...

Extraction of unconjugated bilirubin from serum.

Albumin-bilirubin binding mechanism.

After binding of bilirubin to human serum albumin (1:1), a train of relaxational changes of conformation takes place. The late part of these processes, occurring in the time interval 1-500 s, has been studied by recording the changes of light absorption. Similar processes have been demonstrated after binding of fatty acid anion to the bilirubin-albumin complex as well as after a pH-jump from 6 ...

Interactions of unconjugated bilirubin with bile salts.

The rate of peroxidation of unbound, unconjugated bilirubin (UCB) was used to assess the interactions of UCB with four taurine-conjugated bile salts at pH 8.2, 37 degrees C, and an ionic strength of 0.15. Each of the four structurally different bile salts markedly decreased the rate of peroxidation of UCB in the presence of horseradish peroxidase (HRP); 30% of UCB was bound even at low, premice...